Regulatory

Part:BBa_K4708001:Design

Designed by: Olivia Gibson   Group: iGEM23_BostonU-HW   (2023-10-05)


pLac/RhlI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is constitutively on in the absence of LacI so to work as an indicator of quorum signaling, LacI must be present in the cell. LacI may be in the native genome of the cell, but we recommend inserting a plasmid with LacI to have stronger expression.


Source

Promoter sourced from "Emergent genetic oscillations in a synthetic microbial consortium" (Chen, et.al. Science 2015).

References

Ye Chen et al., Emergent genetic oscillations in a synthetic microbial consortium. Science 349, 986-989(2015). DOI:10.1126/science.aaa3794